top of page
Search

Oocyte Retrieval and Handling: Techniques and Protocols

  • Writer: Sakshi Goswami
    Sakshi Goswami
  • Aug 11
  • 2 min read
ree

Oocyte retrieval is a critical step in the IVF process, involving the collection of mature eggs from the ovaries. This blog explores the techniques, protocols, and best practices for oocyte retrieval and handling in the embryology lab.


The Oocyte Retrieval Process


  1. Stimulation Phase: Controlled ovarian stimulation is performed using medications to promote the development of multiple follicles.

  2. Triggering Ovulation: Human Chorionic Gonadotropin (hCG) or GnRH agonist is administered to mature oocytes and trigger ovulation.

  3. Retrieval Procedure: Oocytes are collected using a trans vaginal ultrasound-guided aspiration technique. The procedure is minimally invasive and performed under sedation.


Handling Oocytes in the Lab


  1. Collection and Identification: Follicular fluid is collected and examined under a microscope to identify oocytes.

  2. Washing and Grading: Oocytes are washed in culture media to remove debris and are graded based on maturity (e.g., germinal vesicle, metaphase I, metaphase II).

  3. Culture: Mature oocytes (MII) are placed in specialized culture media and incubated at optimal conditions.


Protocols for Oocyte Handling


  1. Temperature Control: Maintain oocytes at 37°C to avoid thermal stress.

  2. Minimized Light Exposure: Protect oocytes from photo damage by limiting light exposure during handling.

  3. Sterile Techniques: Use sterile tools and media to prevent contamination.

  4. Documentation: Record all procedures, observations, and outcomes for quality control.


Challenges and Solutions

ree

  1. Fragile Oocytes: Handling oocytes with care is crucial due to their sensitivity to mechanical and environmental stress.

  2. Variability in Response: Not all patients respond uniformly to stimulation, requiring personalized protocols.

  3. Contamination Risk: Strict adherence to aseptic techniques minimizes contamination.


Significance in IVF Success

The quality and handling of oocytes directly influence fertilization rates, embryo quality, and overall IVF outcomes. Proper training and adherence to protocols are vital for embryologists.


References

  1. Van Blerkom, J., & Henry, G. (1992). Oocyte and embryo quality: the role of cytoplasmic factors. Human Reproduction, 7(8), 1283-1292. Link

  2. ESHRE Guidelines. (2021). Oocyte Retrieval Techniques. European Society of Human Reproduction and Embryology. ESHRE

 
 
 

Comments

bottom of page